Journal: eLife

Article Title: Identification of a weight loss-associated causal eQTL in MTIF3 and the effects of MTIF3 deficiency on human adipocyte function

doi: 10.7554/eLife.84168

Figure Lengend Snippet: ( A ) Violin plot of MTIF3 expression in subcutaneous adipose tissue for rs1885988 from Genotype-Tissue Expression (GTEx) Project eQTL. ( B ) Same as in ( A ), but for rs67785913. ( C ) Representative Sanger sequencing traces of rs67785913 CTCT/CTCT and CT/CT clones obtained after CRISPR/Cas9-mediated allele editing and single-cell cloning. ( D ) Normalized Z -score plot of luciferase reporter assays using vectors carrying different DNA fragments of the MTIF3 gene cloned into pGL4.23 luciferase reporter vector. Hypothesis testing was performed by comparing the transcriptional enhancer activity of each of the 12 vectors (F1–12) to the empty vector (minP). All data were plotted as mean ± standard deviation (SD), n = 4 independent experiments, p values are presented in each graph; ordinary one-way analysis of variance (ANOVA) was used for statistical analysis. ( E ) Relative MTIF3 expression (mRNA) in rs67785913 allele-edited cells 2 days before, at, or 2 days post-differentiation induction (day −2, 0, and 2, respectively). n = 3 clonal populations for CTCT/CTCT genotype, n = 5 clonal populations for CT/CT genotype, error bars show SD. ( F ) as in ( E ), but for GTF3A (mRNA) expression. Two-tailed Student’s t -test was used; p values are presented in each graph.

Article Snippet: Sequence-based reagent , Taqman assay for GTF3A , Thermo Fisher Scientific , Hs00157851_m1 , .

Techniques: Expressing, Sequencing, Clone Assay, CRISPR, Cloning, Luciferase, Plasmid Preparation, Activity Assay, Standard Deviation, Two Tailed Test

Journal: eLife

Article Title: Identification of a weight loss-associated causal eQTL in MTIF3 and the effects of MTIF3 deficiency on human adipocyte function

doi: 10.7554/eLife.84168

Figure Lengend Snippet:

Article Snippet: Sequence-based reagent , Taqman assay for GTF3A , Thermo Fisher Scientific , Hs00157851_m1 , .

Techniques: Recombinant, Plasmid Preparation, Sequencing, TaqMan Assay, DNA Extraction, Isolation, Sample Prep, Picogreen Assay

Journal: Cell

Article Title: Microenvironment drives cell state, plasticity, and drug response in pancreatic cancer

doi: 10.1016/j.cell.2021.11.017

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: In brief, digested cells were seeded in 3-dimensional (3D) Growth-factor Reduced Matrigel (Corning), fed with human complete organoid medium containing Advanced DMEM/F12 (GIBCO), 10 mM HEPES (GIBCO), 1x GlutaMAX (GIBCO), 500 nM A83-01 (Tocris), 50 ng/mL mEGF (Peprotech), 100 ng/mL mNoggin (Peprotech), 100 ng/mL hFGF10 (Peprotech), 10 nM hGastrin I (Sigma), 1.25 mM N-acetylcysteine (Sigma), 10 mM Nicotinamide (Sigma), 1x B27 supplement (GIBCO), RSPONDIN-1 conditioned media 10% final, WNT3A conditioned media 50% final, 100 U/mL penicillin/streptomycin (GIBCO), and 1x Primocin (Invivogen) , and maintained at 37°C in 5% CO 2 .

Techniques: Polymer, Recombinant, Amplification, Reverse Transcription, Membrane, DNA Library Preparation, Picogreen Assay, Software, Imaging

Journal: Journal of biomedical and life sciences

Article Title: Poverty Status at Birth Predicts Epigenetic Changes at Age 15

doi: 10.31586/jbls.2024.989

Figure Lengend Snippet: Bivariate Correlations

Article Snippet: For the DNA methylation analysis, approximately 500 ng of genomic DNA, quantified using the Quant-iT Picogreen dsDNA Assay Kit, underwent bisulfite conversion utilizing the EZ-96 DNA Methylation Kit (Zymo Research).

Techniques: